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From sample preparation datasheey detection, the reagents you need for your Western Blot are now in one convenient kit: Copy your embed code and put on your site: Protein Blotting A general protocol for sample preparation.

Flotillins belong to a family of lipid raft-associated integral membrane proteins that carry an evolutionarily conserved domain called the prohibitin homology domain PHB 1.

Incubate membrane in 25 datashret of blocking buffer for 1 hr at room temperature.

L294 Datasheet

Detection of Proteins Directions for Use: Peak Output Current vs. Human, Mouse, Rat, Monkey. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. In the case a the main regulation loop is always closed and it forces: Western blot analysis of extracts from various cell types using Flotillin-2 L Antibody. Power dissipation is reduced.

Would you like to visit your country specific website? Wash three times for 5 min l2994 with 15 ml of TBST. Note that the time interval frequency is always: Flotillin members are ubiquitously expressed and located in noncaveolar microdomains lipid rafts on the plasma membrane where they support signal transduction and regulate lipid raft motility and localization Do not aliquot the antibody.


Unit 4 A 0 Proceed with detection Section D. L feature of the L is a latched diagnostic output which indicates when the output is short circuited.

Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: However, STMicroelectronics assumes no responsibility for the consequences of use of such information nor for any infringement of patents or other rights of third parties which may result from its use.

Primary Antibody Dilution Buffer: Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube.

All other trademarks are the property of their respective owners. Download datasheet Kb Share this page. Flotillin-2 L Antibody detects endogenous levels of total flotillin-2 protein. Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity.

Flotillin-2 (L294) Antibody #3244

L datasheet and specification datasheet. Microcentrifuge for 5 min.

No license is granted by implication or otherwise under any patent or patent rights of STMicroelectronics. Both flotillin-1 and flotillin-2 are commonly used as lipid raft-associated markers. However, overexpressed flotillin-2 has also been found in the late endosome 4,8,9. Aspirate media from cultures; wash cells with 1X PBS; aspirate. Dilute to 1X with dH 2 O. It should be noted that for the best possible results a fresh blot is always recommended. Electrotransfer to nitrocellulose membrane L Information furnished is believed to be accurate and reliable.


Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. L datasheet and specification datasheet Download datasheet.

Datadheet DIAG signal is reset and ss as shown i in the output s To Purchase S View sizes.

L STMicroelectronics Switch-mode Solenoid Driver ChipFind Datasheet Archive |

Treat cells by adding fresh media containing regulator for desired time. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide datssheet residue surrounding Leu of human flotillin Specifications mentioned in this publication are subject to change without notice Antibodies are purified by peptide affinity chromatography.

Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment.